Leptospiral Selection, Growth, and Virulence in Synthetic Medium

Leptospiral selection, growth, and virulence in synthetic medium.

Stalheim, O. H. V. (National Animal Disease Laboratory, Ames, Iowa). Leptospiral selection, growth, and virulence in synthetic medium. J. Bacteriol. 92:946-951. 1966.-The need for protein in leptospiral cultural medium may be circumvented by the use of strains which tolerate the lytic activity of polyoxyethylene sorbitan monooleate (Tween 80), a relatively nonlytic source of essential fatty aci...

Antileptospiral activity of serum. II. Leptospiral virulence factor.

A definite relationship exists between the resistance of leptospires to the antibody-complement system and virulence. Leptospires capable of producing either lethal or renal infections in hamsters or guinea pigs were resistant to the leptospiricidal action of antibody and complement. Avirulent leptospires, in contrast to the virulent organisms, were rapidly immobilized and killed by these serum...

The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence

Pathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity...

Growth and differentiation of human and murine erythroleukemia cell lines in serum-free synthetic medium.

Only two chemicals (transferrin and selenium dioxide) are required to supplement serum-free Roswell Park Memorial Institute Medium 1640 for long-term growth and for spontaneous and induced differentiation of established lines of human and mouse erythroleukemia cells. We describe here two serum-free media (a minimal synthetic medium and a high-density synthetic medium) that support the growth an...

The Growth of Human Tubercle Bacilli, H37, in Synthetic Medium with and without Agar.